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KMID : 0380220060390040371
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Journal of Biochemistry and Molecular Biology
2006 Volume.39 No. 4 p.371 ~ p.376
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Anti-CHH Antibody Causes Impaired Hyperglycemia in Penaeus monodon
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Treerattrakool Supattra
Udomkit Apinunt
Panyim Sakol
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Abstract
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Crustacean hyperglycemic hormone (CHH) plays a major role in controlling glucose level in the haemolymph and also triggers important events during molting and reproductive cycles. In Penaeus monodon, three types of CHH, namely Pem-CHH1, Pem-CHH2 and Pem-CHH3, have been previously characterized. In this study, mouse polyclonal antibody was raised against recombinant Pem-CHH1 that was expressed in Escherichia coli. The anti-Pem-CHH1 antibody recognized all three types of Pem-CHHs but did not cross-react with either related hormone, moltinhibiting hormone of P. monodon, or unrelated human growth hormone. The hyperglycemic activity in the extract from the eyestalk neural tissues was significantly depleted after incubating with anti-Pem-CHH antibody. Direct injection of the antibody into shrimp caused about 30-50% reduction in the haemolymph glucose level. The result demonstrates the ability of anti-Pem-CHH1 antibody to deplete the activity of CHH in vivo, and thus provides a possibility of using anti-Pem-CHH1 antibody to inhibit the hormone activity as a strategy to modulate growth and reproduction in this species.
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KEYWORD
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Antibody, Black tiger shrimp, Crustacean hyperglycemic hormone, Eyestalk, Sinus gland
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